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Bacteriology, Mycology, Virology I

Course developers

Prior knowledge

Vete4035, Cytology, Histology and Embryology I

Course abstract

During the course of studies students are acquainted with agents of infectious diseases in various species of animals - structure of microorganisms, their morphological, biochemical and biological qualities, multiplication, growing, classification and their connection with various processes of circulation in nature. Students learn the general laboratory methods for testing of bacteria.

Learning outcomes and their assessment

Knowledge about infectious diseases in various species of animals, structure of bacteria, fungi and viruses), their morphological, biochemical qualities, their growing and general bacteriological diagnosis. 3 teoretical tests.
Skills to perform without assistance diagnostics of conditional pathogens at the laboratory, to analyze and do interpretation of results given. Practical works.
Competence to evaluate the results of the investigation in the study process and to explain the action in the given situation. 3 practical tests.

Course Content(Calendar)

1.Lecture – 2 h; Introduction. History of Microbiology. Classification and nomenclature of Bacteria. / Pract.W. - 2 h; Safety rules in the microbiology laboratory. Microscope and microscopy.
2. Lecture – 2 h; Bacterial cells forms and size, bacterial cell structure. / Pract.W. - 2 h; Smear preparation and simple staining.
3. Lecture – 2 h; Bacterial growth and metabolism. / Pract.W. – 2 h; Gram staining method.
4. Lecture – 2 h; Cultivation of bacteria. / Pract.W. – 2 h; Motility determination of bacteria.
5. Lecture – 1st TEST. The test samples, 2 h; / Pract.W. – 2 h; Acid-fast, spore and capsule forming bacteria staining.
6. Lecture – 2 h; Investigation scheme of bacteriological material. / Pract.W. - 2 h; Submission form. Primary isolation of bacteria.
7. Lecture – 2 h; Physical factor impact on growth of microorganisms. / Pract.W. - 2 h; Bacteria pure culture technique.
8. Lecture – 2 h; Chemical factor impact on growth of microorganisms. / Pract.W. - 2 h; Sterilization and Disinfection.
9. Lecture – 2 h. Bacterial biochemical identification kits; / Pract.W. - 2 h; results interpretation.
10. Lecture – 2 h; 2nd TEST. Biological control factors of bacteria; / Pract.W. – 2 h; Bacterial growth
11. Lecture – 2 h; Antibiotic resistance of bacteria. Antibacterial mechanism. / Pract.W. – 2 h Antibiogramm.
12. Lecture – 2 h; Pathogenity and virulence of bacteria. / Pract.W.– 2 h; Assessment and interpretation of antibiogramm results.
13. Lecture – 2 h; Biomembrane and animal normal microbiota. / Pract.W. – 2 h; Air and environment microbial analysis. Hand and mouth microbial analysis.
14. Lecture – 2 h; Bacteriological material investigation scheme. / Pract.W. – 2 h; Assessment and interpretation results of hand, mouth, air and environment microbial analysis.
15. 3rd TEST. Transmission of infectious agents and Biosecurity. / Lecture – 2 h; Pract.W. – 2 h;

Requirements for awarding credit points

Attendance for lectures is not compulsary. Attendace for practical classes of at least 90% is required.
Students are required to PASS three theoretical and three practical tests.
Failed theoretical tests are required to be retaken until the next test.
Final grade/ assignment - PASS with no mark.
Students are required to carry out any missed practical classes in two weeks time.
Failure to meet the two week deadline constitutes an additional theoretical assignment on the specific topic.
Failure to PASS all assignments and overdue assignments of the study course in the deadline of an academic year constitutes retaking all assignments and/or tests.

Description of the organization and tasks of students’ independent work

Literature, lecture and praktical work material studies, analysis of praktical work results.

Criteria for Evaluating Learning Outcomes

Student skills are assessed in accordance with the procedures specified in the tasks of the practical work.
The study course tests are evaluated in accordance with the criteria of the 10-point system scale indicated in the LLU Study Regulations.
In order to get the final grade of the study course "passed", all tests must be evaluated with a successful grade.

Compulsory reading

1. Songer J.G., Post K. W. Veterinary Microbiology: Bacterial and Fungal Agents of Animal Disease. Missouri: Elsevier Saunders, 2005. 448 p.
2. Harvey R.A., Champe P.C. Microbiology. 2nd ed. Philadelphia: Lippincott Williams & Wilkins, 2007. 438 p.
3. Clinical veterinary microbiology. B. Markey, F. Leonard, M. Archambault, A. Cullinane, D. Maguire. 2nd ed. USA: Mosby-Elsevier, 2013. 915 p.
4. Scott McVey D., Kennedy M., Chengappa M.M. Veterinary Microbiology. Oxford: Wiley-Blackwell, 2013. 648 p.
5. Veterinary microbiology and microbial diseases. P.J. Quinn, B.K. Markey, F.C. Leonard, P.E.S. Fitz, S. Fanning, P.J. Hartigan. Oxford: Wiley-Blackwell, 2011. 656 p.

Further reading

Leboffe M.J., Pierce B.E. A Photographic Atlas for the Microbiology laboratory. 4th ed. Morton Pub., 2011. 266 p.
Pieejams: http://www.uobabylon.edu.iq/eprints/publication_12_24710_198.pdf

Periodicals and other sources

1. Videokasete “Baktēriju vairošanās”.

Notes

Compulsory course of study programme Veterinary medicine