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Course title	Bacteriology, mycology, virology I
Course code	VeteB011
Credit points (ECTS)	4
Total Hours in Course	108
Number of hours for lectures	26
Number of hours for seminars and practical classes	0
Number of hours for laboratory classes	26
Independent study hours	56
Date of course confirmation	20/12/2023
Responsible Unit	Institute of Food and Environmental Hygiene
Course developers
Dr. med. vet., prof. Anda Valdovska Dr. med. vet., doc. Gundega Gulbe Ph.D., doc. Daiga Gāliņa First-cycle professional higher education(līm.), pasn. Endija Maraka First-cycle professional higher education(līm.), pasn. Madara Beāte Balode
Prior knowledge
BiolB004, Animal Biology, Ecology and Ethology II VeteB007, Cell biology, Histology I
Course abstract
During this study course, students learn about the structure of bacteria, their morphological, biochemical and biological characteristics, reproduction, classification and control methods (physical, chemical and biological). Students learn the general laboratory methods for bacterial identification.
Learning outcomes and their assessment
Knowledge about the structure of bacteria, their morphological, biochemical qualities, their growth conditions and bacteriological identification methods. Skills to perform (without assistance) general laboratory methods for the identification and/or differentiation of bacteria. Competence to evaluate the obtained test results and to be able to explain them. Acquired knowledge is assessed with 3 theoretical and 3 practical tests.
Course Content(Calendar)
1st week: Lecture – Introduction. History of microbiology. Classification and nomenclature of bacteria. 2 h Laboratory work – Plan of the semester. Safety aspects of working in laboratory. Microscope and microscopy. 2 h 2nd week: Lecture – Size and forms of bacteria, the structure of bacteria. 2 h Laboratory work – Smear preparation and simple staining. 2 h 3rd week: Lecture – Metabolism and reproduction of bacteria. 2 h Laboratory work – Gram’s staining method. 2 h 4th week: Lecture – Cultivation of bacteria. 2 h Laboratory work – Staining of capsules, spores and acid-fast bacteria. 2 h 5th week: Lecture – 1st theoretical test. Physical methods of microbial control. 2 h Laboratory work – 1st practical test. Motility detection in bacteria. 2 h 6th week: Lecture – Chemical methods of microbial control. 2 h Laboratory work – Sterilization and disinfection. 2 h 7th week: Lecture – Samples and sample submission to the laboratory for bacteriological investigation. 2 h Laboratory work – Sample submission form. Primary isolation of bacteria from a sample. 2 h 8th week: Lecture – 2nd theoretical test. Bacteriological day-by-day examination plan and 5i approach. 2 h Laboratory work – Pure culture isolation. 2 h 9th week: Lecture – Biological methods of microbial control. The mechanism of action of the antimicrobials (I). 2 h Laboratory work – Colony morphology evaluation. 2 h 10th week: Lecture – The mechanism of action of the antimicrobials (II). 2h Laboratory work – 2nd practical test. Rapid biochemical tests. 2 h 11th week: Lecture - Mechanisms of antibiotic resistance. 2 h Laboratory work – Inoculation of biochemical tests. Antibiogram. 2 h 12th week: Lecture – Bacterial pathogenicity and virulence. 2 h Laboratory work – Evaluation and interpretation of biochemical test results. Evaluation and interpretation of antibiogram. 2 h 13th week: Lecture – Biomembranes and normal microbiota of animals. 1h, 3rd theoretical test. 1h Laboratory work – 3rd practical test. 2 h
Requirements for awarding credit points
Lecture attendance is not mandatory. Laboratory work attendance is mandatory. Each student is allowed to miss 3 laboratory works in a semester (including two excused absences). Missing more than 3 laboratory works constitutes retaking the whole study course. Students are required to carry out any missed practical works in two weeks’ time. Failure to meet the two week deadline constitutes an additional theoretical assignment on the specific topic. Students are required to PASS three theoretical and three practical tests. Failed theoretical tests are required to be retaken until the next test. Each theoretical test can be taken three times – two times in a written form, and the third time with a committee. Not passing a theoretical test with the third time constitutes retaking the whole study course. Final grade – PASS with no mark. Failure to PASS all assignments and overdue assignments of the study course within the period of individual studies and examinations constitutes retaking the whole study course.
Description of the organization and tasks of students’ independent work
Studies of literature, lecture and practical work materials, analysis of practical work results.
Criteria for Evaluating Learning Outcomes
Student skills are assessed in accordance with the procedures specified in the tasks of the practical work. The study course tests are evaluated in accordance with the criteria of the 10-point system scale indicated in the LBTU Study Regulation. In order to get the final grade “passed”, all theoretical and practical tests must be passed.
Compulsory reading
1. B. Markey, F. Leonard, M. Archambault, A. Cullinane, D. Maguire (2013) Clinical veterinary microbiology. 2nd ed. USA: Mosby-Elsevier. 915 p. 2. Scott McVey D., Kennedy M., Chengappa M.M. (2022) Veterinary Microbiology. 4th ed. Hoboken, NJ: John Wiley & Sons, Inc. 879 p. 3. P.J. Quinn, B.K. Markey, F.C. Leonard et al (2016) Concise Review of Veterinary Microbiology. 2nd ed. Oxford, UK: John Wiley & Sons, Inc. 208 p. 4. P.J. Quinn, B.K. Markey, F.C. Leonard, P.E.S. Fitz, S. Fanning, P.J. Hartigan (2011) Veterinary microbiology and microbial diseases Oxford: Wiley-Blackwell. 656 p.
Further reading
Leboffe M.J., Pierce B.E. A Photographic Atlas for the Microbiology laboratory. 4th ed. Morton Pub., 2011. 266 p. Pieejams: http://www.uobabylon.edu.iq/eprints/publication_12_24710_198.pdf
Notes
Compulsory course in second cycle professional higher education study programme “Veterinary medicine” in the 3rd semester