LV EN RU DE FR

Bacteriology, Mycology, Virology III

Course developers

Prior knowledge

Vete2024, Bacteriology, Mycology, Virology II

Course abstract

During the study course students get acquainted with the infectious diseases of different species of animals - the structure of viruses and fungi, their morphological and biological properties, reproduction, systematics. To get acquainted with the pathogenesis of pathogenic fungi and viruses proposed in the susceptible macroorganism, the clinical manifestation and diagnosis of the diseases caused by them. Students learn laboratory methods for detection of pathogenic fungi and viruses.
In the molecular biology section, students will be with the components and structure of genetic material, history of molecular biology, main processes of molecular biology and the main methods and applications of molecular biology.

Learning outcomes and their assessment

Knowledge of the morphology, biochemical characteristics, growth and general bacteriological diagnosis of animal infectious agents. Knowledge about the pathogenesis of infection in a susceptible macroorganism, its clinical manifestation. Knowledge of types, components and structure of genetic material. An understanding of the basic processes and methods of molecular biology. Basic knowledge of main molecular biology laboratory methods and applications. 3 tests
Skills: to independently diagnose in a laboratory the fungal pathogens as infectious agents, to analyze and interpret the results. Ability to apply the acquired knowledge about the genome and the processes of realization of genetic information in the evaluation of the impact of mutations. Practicals
Competence to evaluate laboratory results obtained in virology and mycology, to be able to explain them to the sample sender. The ability to relate specific stages of cell development or processes within the cells to molecular biology processes.
KEGA work (in the bacteriology or mycology field).

Course Content(Calendar)

1. Lecture: General features of fungi. Practical class: Biosafety in Lab. Preparation of smear. / 2 h lecture and 2 h Pract. work;
2. Lecture: Aspergillus, Penicilium spp. and Zygomycetes. Practical class: Laboratory diagnostic of Aspergillus, Penicilium spp. and Zygomycetes. / 2 h lecture and 2 h Pract. work
3. Lecture: Pathogenic yeasts. Practical class: Laboratory diagnostic of dimorphic fungi. / 2 h lecture and 2 h Pract. work.
4. Lecture: Dermatophytes. Practical class: Laboratory diagnostic of Dermatophytes. / 2 h lecture and 2 h Pract. work.
5. The 1st TEST. Lecture: Nature, structure, taxonomy and morphology of virusies. Practical class: Safety in virology Lab. Virological examination methods. / 2 h lecture and 2 h Pract. work;
6. Lecture: Replication of viruses. Practical class: Microscopy methods of viruses; formation of inclusions. / 2 h lecture and 2 h Pract. work.
7. Lecture: Characteristics of viruse’s family (1-4). Practical class: Cell cultures in virology. / 2 h lecture and 2 h Pract. work.
8. Lecture: Characteristics of viruse’s family (5-6). Practical class: Diagnostic serology. / 2 h lecture and 2 h Pract. work.
9. Lecture: Virus and physical-chemical factors. Antiviral chemotherapy. Practical class: The 2nd TEST / 2 h lecture and 2 h Pract. work.
10. Lecture: History of molecular biology and the most important discoveries. Application of molecular biology methods. Practical class: DNA extraction / 2 h lecture and 2 h Pract. work.
11. Lecture: Genetic material. Composition and spatial structure. Practical class: nucleus and chromosomes (microscopy) / 2 h lecture and 2 h Pract. work.
12. Lecture: Protein synthesis. Practical class: cell division (microscopy). / 2 h lecture and 2 h Pract. work.
13. Lecture: Cell division, cell cycle, apoptosis. Practical class: gametogenesis (microscopy). / 2 h lecture and 2 h Pract. work.
14. Lecture: Transport of substances, signaling pathways. Practical class: osmosis (microscopy). / 2 h lecture and 2 h Pract. work.
15. Lecture: Methods of molecular biology. Detailed review of the most important methods, application examples. Practical class: PCR and electrophoresis. The 3rd TEST / 2 h lecture and 2 h Pract. work.

Requirements for awarding credit points

Attendance for lectures is not compulsary. Attendace for practical classes of at least 90% is required.
Students are required to PASS four theoretical tests and one clinical and epidemiological case analysis work (KEGA).
Failed theoretical tests are required to be retaken until the next test.
Students are required to carry out any missed practical classes in two weeks time.
Failure to meet the two week deadline constitutes an additional theoretical assignment on the specific topic.
Failure to PASS all assignments and overdue assignments of the study course in the deadline of an academic year constitutes retaking all assignments and/or tests.
Final test – written exam with a mark.
A student can take an exam in session if all the tests and KEGA work have been completed to the beginning of the individual study and examination period (session).

Description of the organization and tasks of students’ independent work

Studies of literature, lecture and practical work material, analysis of practical work results.
KEGA purpose: to master the use of theoretical knowledge in practical situations demonstrating plan organizing skills for sample microbial examination, to isolate and identify conditionally pathogenic bacteria and microscopic fungi from samples. KEGA objectives: interpret information from the sample submission form, compile a sample investigation scheme, carry out sample bacteriological and/or mycological examination and fill out the examination protocol. KEGA assignment (identification protocol and sample submission form) is to be submited in three weeks time from the start of the examination, but not later than three weeks before the ending of semester. Students orally present their KEGA assignments individually in the presence of a member of the teaching staff. The KEGA assignment can be initiated if the student has passed the study course part „Bacteriology, Mycology, Virology II”. The student must succesfully pass the tests in specific part of the study course in order to started the KEGA assignment.

Criteria for Evaluating Learning Outcomes

Students' knowledge and skills are assessed in accordance with the procedures specified in the tasks of the practical works.
To take the exam, all tests must be successfully completed. The assignment of KEGA is graded with „passed”.
All tests and answers of exam are evaluated in accordance with the criteria of the 10-point system scale indicated in the LLU Study Regulations.
To take the exam, all tests must be successfully completed. The exam is organized in writing - in the form of a test (100 questions with multiple choice answers).

Compulsory reading

1. Songer J.G., Post K.W. Veterinary Microbiology: Bacterial and Fungal Agents of Animal Disease. Missouri: Elsevier Saunders, 2005. 448 p.
2. Harvey R. A., Champe P.C. Microbiolgy. 2nd ed. Baltimore: Lippincott Williams & Wilkins, 2007. 438 p.
3. Clinical veterinary microbiology. B. Markey, F. Leonard, M. Archambault, A. Cullinane, D. Maguire. 2nd ed. USA: Mosby-Elsevier, 2013. 915 p.
4. Scott McVey D., Kennedy M., Chengappa M. M. Veterinary Microbiology. Oxford: Wiley-Blackwell, 2013. 648 p.
5. Veterinary microbiology and microbial diseases. P.J. Quinn, B.K. Markey, F.C. Leonard, P.E.S. Fitz, S. Fanning, P.J. Hartigan. Oxford: Wiley-Blackwell, 2011. 656 p.

Notes

Compulsory course of study programme Veterinary medicine