Course code Ķīmi3015

Credit points 3

The Basics of Biochemistry

Total Hours in Course81

Number of hours for lectures16

Number of hours for laboratory classes16

Independent study hours49

Date of course confirmation30.03.2021

Responsible UnitInstitute of Food

Course developer

author Pārtikas institūts

Ingmārs Cinkmanis

Dr. sc. ing.

Prior knowledge

Ķīmi1012, Chemistry

Course abstract

Students will obtain knowledge, skills and competence on biochemical transformations, their catalysis with enzymes, regulations of transformations by information flow through biochemical signalling, as well as on matter and energy flows through metabolism that will give rise to complexity of life and will form the base for further studies of physiology of animals.

Learning outcomes and their assessment

Extensive knowledge about properties and structure of lipids, carbohydrates and proteins in the animal nutrition. Biochemical processes, metabolism and the biochemical regulation in the animal body – 1., 2., 3., and 4. Test.
The skills to make in use the theoretical knowledge on pathways of metabolism and their regulation in tissues cells in the further specialization studies and in solving professional problems will be developed during biochemistry studies. These skills assist to create scientifically based hypothesis and their possible solutions on the base of empirical data and recent theoretical cognitions - laboratory works. The competence to independently acquire, select and analyze information on lipids, carbohydrates and protein metabolism in animals and their nutrition. To use information, to make and explain decisions and solve scientific and professional issues - Test with a mark.

Course Content(Calendar)

1. Lecture: Introduction. Simple and conjugated proteins. Nucleic Acids
2. Lecture: Enzymes / Instruction of laboratory rules and safety 1.Lab work: The starch hydrolysis with amylase and enzyme substrate specificity - 2h.
3.Lecture: Biological oxidation
4. Lecture: Carbohydrate metabolism I / 1.Test / 2. Lab Work: Evaluation of tissue respiratory intensity - 2h
5. Lecture: Carbohydrate metabolism II
6. Lecture: Fermentation processes / 3. Lab work: Determination of milk lactose - 2h
7. Lecture: Lipid metabolism I
8. Lecture: Lipid metabolism II / 2. Test / 4. Lab. work: Properties of pancreas lipase and bile - 2h
9. Lecture: Protein metabolism I
10. Lecture: Protein metabolism II / 5. Lab.work: Protein enzymatic hydrolysis and 6. Lab. work: Gastric juice - 2h
11. Lecture: Nucleic acid and nucleotide metabolism.
12. Lecture: Gene / 3. Test / 7. Lab work: Yeast nucleoprotein hydrolysis - 2h
13. Lecture: Biochemistry of muscle and connective tissue
14. Lecture: Micro and macro elements. / 8. Lab. work: Muscle tissue - 2h
15. Lecture: Water soluble vitamins
16. Lecture: Fat-soluble vitamins / 4. Test

Requirements for awarding credit points

8.laboratory works and 4 tests with no lower mark in tests than 4 points.

Description of the organization and tasks of students’ independent work

Preparation of laboratory reports - compilation and systematization of chemical reactions, observation, conclusions and experimental progress.

Criteria for Evaluating Learning Outcomes

It is necessary to write successfully all tests, i.e. no lower mark than 4 points. All laboratory work protocols must be checked and completed. Test with a mark.

Compulsory reading

1. Miķelsone, V. Bioķīmija: mācību līdzeklis. Jelgava: LLU, 2008. 197 lpp.
2.Morozovs A., Jākobsone I., Mekšs P. Pārtikas ķīmija. Rīga: LU Akadēmiskais apgāds, 2008. 208 lpp.
2. Garrett R.H., Grisham C.M. Biochemistry. Australia etc.: Thomson Books/Cole, 2005, p.1086-1169.
4. Comprehensive glycoscience: from chemistry to systems biology. Vol. 3. Biochemistry of glyco-conjugate glycans. Carbohydrate-mediated interactions. Editor-in-chief J.P. Kamerling. Oxford: Elsevier, 2007. 902 p.

Further reading

1. Fry M. Essential Biochemistry for Medicine. Oxford: Wiley-Blackwell, 2010. 308 p.
2. Handbook of Biochemistry and Molecular Biology. 4th Edition. Edited by Rogur L. Lundblad and Fiona M . MacDonald. CRC Press 2010, eBook ISBN: 978-1-4200-0869-2. Pieejams LLU FB datubāze CRCnetBase https://www.taylorfrancis.com/books/e/9781466515154
3. Lipoic Acid. Energy Production, Antioxidant Activity and Health Effects. Edited by M.S.Patel and L.Pack. CRC Press 2008, eBook ISBN: 978-1-4200-4539-0 Pieejams LLU FB datubāze CRCnetBase http://www.crcnetbase.com/doi/book/10.1201/9781420045390

Periodicals and other sources

1. Journal of Animal Science. American Society of Animal Science. Print ISSN 0021-8812, Online ISSN 1525-3163.

Notes

Full and part time professional Bachelor of Agricultural Sciences, qualification Zootechnics in Breeding